Open Access

Downloads

Download data is not yet available.

Abstract

Dendrobium is one of the largest and most important orchid genera because of their ornamental and commercial value. Plant tissue culture is an established method for the effective micropropagation of of valuable plants. In this study, Dendrobium caesar keikis were sterilized with sodium hypochlorite 2.5 % in 15 minutes. Effects of 3 kinds of cytokinin (TDZ, BA and kinetin), adenine and 3 kinds of auxin (IAA, IBA, NAA) with different concentrations on inducing shoots and roots were studied. The highest shoot initation was observed on Knudson C (KC) medium supplemented with BA 2.0 mg/L. Strong roots were induced in KC media supplied with 0.5 g/L active charcoal (AC) and 1.0 mg/L IAA. In order to transfer in vitro plant to the nursery garden, the highest percentage of survival seedling was shown on coconut husk substrate, and peanut husk was the best for the growth and development of Dendrobium caesar. Micropropagation and nursery at garden for Dendrobium caesar were successfully established.



Author's Affiliation
Article Details

Issue: Vol 2 No 3 (2018)
Page No.: 14-22
Published: May 23, 2019
Section: Original Research
DOI: https://doi.org/10.32508/stdjns.v2i3.748

 Copyright Info

Creative Commons License

Copyright: The Authors. This is an open access article distributed under the terms of the Creative Commons Attribution License CC-BY 4.0., which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 How to Cite
Pham, Q., Duong, K., Quach, P., & Hoang, M. (2019). Micropropagation and nursery at garden of Dendrobium caesar. Science and Technology Development Journal - Natural Sciences, 2(3), 14-22. https://doi.org/https://doi.org/10.32508/stdjns.v2i3.748

 Cited by



Article level Metrics by Paperbuzz/Impactstory
Article level Metrics by Altmetrics

 Article Statistics
HTML = 32 times
Download PDF   = 19 times
Total   = 19 times