Open Access 
Downloads
Abstract
Dendrobium is one of the largest and most important orchid genera because of their ornamental and commercial value. Plant tissue culture is an established method for the effective micropropagation of of valuable plants. In this study, Dendrobium caesar keikis were sterilized with sodium hypochlorite 2.5 % in 15 minutes. Effects of 3 kinds of cytokinin (TDZ, BA and kinetin), adenine and 3 kinds of auxin (IAA, IBA, NAA) with different concentrations on inducing shoots and roots were studied. The highest shoot initation was observed on Knudson C (KC) medium supplemented with BA 2.0 mg/L. Strong roots were induced in KC media supplied with 0.5 g/L active charcoal (AC) and 1.0 mg/L IAA. In order to transfer in vitro plant to the nursery garden, the highest percentage of survival seedling was shown on coconut husk substrate, and peanut husk was the best for the growth and development of Dendrobium caesar. Micropropagation and nursery at garden for Dendrobium caesar were successfully established.
Issue: Vol 2 No 3 (2018)
Page No.: 14-22
Published: May 23, 2019
Section: Original Research
DOI: https://doi.org/10.32508/stdjns.v2i3.748
Copyright: The Authors. This is an open access article distributed under the terms of the Creative Commons Attribution License CC-BY 4.0., which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Download PDF = 740 times
Total = 740 times
Most read articles by the same author(s)
- Phuong Ngo Diem Quach, Tran Ngoc Bao Do, Phuong Dong Tra, Tam Thien Luong, Thu Nguyen Anh Tran, Thuoc Linh Tran, Biological activities and micropropagation of non-vascular plants from Bidoup-Nui Ba National Park, Lam Dong , Science & Technology Development Journal: Natural Sciences: Vol 1 No T1 (2017)
- Trinh Le Phuong Ngo, Thanh Nguyen Chu, Minh Thi Thanh Hoang, Evaluating the salt resistance of Arabidopsis thaliana induced by plant growth-promoting rhizobacteria (PGPR) isolated from Can Gio mangrove forest , Science & Technology Development Journal: Natural Sciences: Vol 1 No T2 (2017)
- Dai Minh Cao, Phuong Thi Bach Vu, Phuong Ngo Diem Quach, Effect of growth factors on the production of biomass of the hairy root of Urena lobata , Science & Technology Development Journal: Natural Sciences: Vol 1 No T3 (2017)
- Phuong Thi Bach Vu, Nhi Thi Yen Trieu, Kien Cong Duong, Phuong Ngo Diem Quach, Micropropagation of Musa balbasiana (BBB group) , Science & Technology Development Journal: Natural Sciences: Vol 2 No 3 (2018)
- Tan Quoc Tran, Tam Thien Luong, Hoang Ngo Phan, Phuong Ngo Diem Quach, Biological activities of the liverwort Marchantia polymorpha L. collected at Da Lat, Lam Dong province , Science & Technology Development Journal: Natural Sciences: Vol 2 No 5 (2018)
- Phuong Thi Bach Vu, Hong Thi Anh Pham, Phuong Ngo Diem Quach, In this study, NaCl at varrious concentrations of 4 – 10 g/L was used to investigate the salt tolerance of in vitro shoot cuttings of Chrysanthemum indicum. Morphological, physiological and biochemical changes during the response of shoot cuttings in the , Science & Technology Development Journal: Natural Sciences: Vol 2 No 4 (2018)
- Thanh Nguyen Chu, Tieng Van Nhut Pham, Le Van Bui, Minh Thi Thanh Hoang, Isolation and screening of Pseudomonas isolates enhance salinity tolerance of peanut (Arachis hypogaea L.) , Science & Technology Development Journal: Natural Sciences: Vol 4 No 1 (2020)
- Phuong Thi Bach Vu, Dai Minh Cao, Phuong Ngo Diem Quach, Effects of some precursors and elicitors on the growth and α-glucosidase inhibitory activity of in vitro Urena lobata L. hairy roots , Science & Technology Development Journal: Natural Sciences: Vol 4 No 3 (2020)
