Micropropagation of Musa balbasiana (BBB group)

Abstract

The banana domestic market as well as global market are growing. Musa balbasiana (BBB group) is now one of the preferred banana types; however, this banana group is facing up to risk of degeneration and disease. Therefore, the micropropagation of banana to provide genetic stability, disease-free seedlings, and enriching the types of bananas in the market is essential. In this study, we have completed the process of Musa balbasiana (BBB group) micropropagation. The study results showed that the samples were sterilized with NaOCl 3% solution in 12-15 minutes depending on the size and age of the samples, buds were regenerated on Murashige and Skoog medium (MS) added 0.5 mg/L 1-phenyl-3 (1,2,3 thiadiazol-5-yl) (TDZ). MS medium supplemented with 1 mg/L benzylaminopurine (BA) and 0.25 mg/L kinetin made high efficiency shoot initation (10.700 ± 0.135 buds/sample) and the shoot height at the day of 20 was 3.023 ± 0.018 cm. Musa balbasiana (BBB group) had been the most induced roots on MS medium without plant growth regulators with 4.533 ± 0.058 roots/shoot and the roots were 2.433 ± 0.067 cm in length. Complete seedlings (with roots, stems, and leave) were transferred to the nursery and planted on sand with the highest survival rate of 91.1 %. After two weeks, the survival plants were moved to grown on clean soil for the best growth and development.