Isolation and establishment of reference standards for resveratrol and isorhapotigenin of the linas of Gnetum montanum markgr

Abstract

Gnetum montanum, commonly known as Dây gắm, belongs to the Gnetaceae family. Chemical composition studies have shown that stilbene compounds are the main constituents of this plant. Notably, resveratrol and isorhapotigenin are two significant compounds, comprising approximately 3-5% of the ethanol extract. Both compounds exhibit remarkable biological activities, including anti-inflammatory, anti-cancer, antioxidant properties, tyrosinase inhibition, Cytochrome P450 inhibition, and α-glucosidase inhibition. Resveratrol and isorhapotigenin are essential for assessing and evaluating the quality of Gnetum montanum liana and related medicinal materials within the Gnetaceae family. This study successfully established reference standards for resveratrol and isorhapotigenin, isolated from Gnetum montanum liana. The results showed high purity levels of 99.14% for resveratrol and 96.38% for isorhapotigenin. The process for determining the purity of resveratrol and isorhapotigenin in Gnetum montanum liana was successfully validated using the HPLC-DAD Agilent 1260 system. The mobile phase used in this process was a gradient of ACN and 0.5% TFA in water. The research covered system compatibility, specificity, linearity range, repeatability, and accuracy, all in accordance with ICH guidelines. Establishing these reference standards is crucial for ensuring consistency and reliability in the quality control processes of Gnetum montanum and related medicinal products. With these highly pure standards, researchers and manufacturers can accurately measure and verify the presence and concentration of resveratrol and isorhapotigenin in various samples. This contributes to the overall efficacy and safety of herbal medicines derived from Gnetum montanum. Furthermore, the validated HPLC-DAD method provides a robust and reliable analytical technique for routine quality control, supporting the standardization of herbal products and enhancing confidence in their therapeutic use. This advancement underscores the importance of rigorous scientific methods in the development and quality assurance of traditional medicinal resources.