Collection of human derived acellular dermal matrix by a combination of ultrasonic technique and sodium dodecyl sulfate solution

Abstract

Extracellular matrix (ECM) from the human skin is a potential source of materials for regenerative medicine in some applications: treatment of skin loss lesions, replacement of lost tissues or 3D bioprinting ink preparation. The ECM could only be used when the cellular component that induced the immune response was removed. Common decellularization methods could be divided into three categories: physical, chemical, and biological. This study showed that these categories could attain optimal when they were combined together rather than be individually used. This paper presented the study in which the human skin was collected and preserved in 1X phosphate buffered saline (PBS) supplemented with antibiotics in 24 hours. After that, the obtained skin samples were decellularized by three methods including using ultrasonic combining with sodium dodecyl sulfate (SDS) solution, hypertonic NaCl solution combining with SDS solution and a trypsin solution at some concentrations. The cell reduction efficiency was assessed by a histological staining and quantification of residual DNA concentrations. The results showed that the ultrasonic combining with SDS completely removed the cellular component and better preserved the structure of the ECM and the ECM was not cytotoxic to human fibroblasts in vitro. The result could be the basis for obtaining the acellular ECM from human skin for biomedical applications.