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Abstract

Quantum dots (QDs) have the potential to be used as a marker in research and supporting for medical treatment because of their unique optical and electronic properties such as size-tuneable light emission, narrow emission, high photostability, etc. With the goal of applying for biomarkers, QDs are often attached with antibodies. In order to simplify the binding process, we experimented to attach adaptor protein, namely protein A/G to CdSe/ZnS QDs covered by 3-mercaptopropionic acid (MPA). 80.7 % and 51.2 % of protein A/G at the concentration of 60 μg/mL and 20 μg/mL, respectively, conjugated with QDs in phosphate buffer saline (PBS) without supporting of N-Ethyl-N’-(3dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS). After attaching to protein A/G, anti-pan T antibody could recognize and visualize Jurkat T cells. In conclusion, protein A/G was conjugated successfully on QDs and initially support for application in cell labeling.



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Article Details

Issue: Vol 2 No 6 (2018)
Page No.: 57-65
Published: Sep 17, 2019
Section: Original Research
DOI: https://doi.org/10.32508/stdjns.v2i6.844

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Creative Commons License

Copyright: The Authors. This is an open access article distributed under the terms of the Creative Commons Attribution License CC-BY 4.0., which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 How to Cite
Tran, H. D. T., Nguyen, C. T., Le, K. T., Vo, N. T. T., & Tran, H. V. (2019). Application of quantum dots as fluorescent maker in cell labeling. Science and Technology Development Journal - Natural Sciences, 2(6), 57-65. https://doi.org/https://doi.org/10.32508/stdjns.v2i6.844

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