Open Access

Downloads

Download data is not yet available.

Abstract

Catharanthus roseus hairy roots induced by Agrobacterium rhizogenes get potential to apply to many fields. The suitable conditions for culturing depend on each hairy root line. In shaken liquid media in dark at 25 oC, VIN002-12 and VIN005-07 hairy root lines had the best growth in hemi-concentrated Gamborg’B5 media while VIN072-15 and VIN077-09 lines showed the best growth in in hemi-concentrated White media. The appropriate initial pH of medium for the lines was in range of 5.7–6.5. The hairy root lines used 2–5% sucrose as an optimal source of carbon for their growth. In selected conditions, the growth kinetics curves showed the end of exponential phase at the 28th day of culture with VIN005-07 and VIN072- 15 lines whereas at the 35th day with VIN002-12 and VIN077-09 lines. The initial results are quite possible to produce biomass for researches on four hairy root lines in the future.


 



Author's Affiliation
Article Details

Issue: Vol 2 No 1 (2018)
Page No.: 5-12
Published: Jan 6, 2019
Section: Original Research
DOI: https://doi.org/10.32508/stdjns.v2i1.667

 Copyright Info

Creative Commons License

Copyright: The Authors. This is an open access article distributed under the terms of the Creative Commons Attribution License CC-BY 4.0., which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 How to Cite
Nhut, N. N., & Le, B. V. (2019). Study on the growth of Agrobacterium rhizogenes-induced Catharanthus roseus hairy roots in liquid media. Science and Technology Development Journal - Natural Sciences, 2(1), 5-12. https://doi.org/https://doi.org/10.32508/stdjns.v2i1.667

 Cited by



Article level Metrics by Paperbuzz/Impactstory
Article level Metrics by Altmetrics

 Article Statistics
HTML = 55 times
Download PDF   = 49 times
Total   = 49 times