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In this paper, plant growth regulators including 6-benzylaminopurine (BA), kinetin, indole-3-acetic acid (IAA), gibberellic acid (GA3) and ethrel, at different concentrations were used individually or in combination to induce adventitious shoots from the explants, which contain shoot apical meristem and young leaves. Histological and physiological changes during shoot development were analysed. The highest shoot initiation was achieved on Murashige and Skoog (MS) medium supplemented with 0.5 mg/L BA and 1.0 mg/L GA3. Regenerated shoots were rooted on MS medium with 0.25 or 0.5 mg/L IAA. Shoot development from in vitro shoot explants initiated from the axil and cortex of stem. The shoot regeneration from shoot apical explants was effected by the meristem integrity or auxin from shoot apical meristem. Roles of plant growth regulators, especially polar auxin transport, and the ablation on the shoot initiation were discussed.

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Issue: Vol 1 No 6 (2017)
Page No.: 58-67
Published: Dec 7, 2018
Section: Original Research

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Copyright: The Authors. This is an open access article distributed under the terms of the Creative Commons Attribution License CC-BY 4.0., which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 How to Cite
Ngo, H., Tran, H., & Bui, V. (2018). Roles of plant growth regulators on the shoot development from the shoot apical meristem of Eustoma grandiflorum (RAF.) shinners. Science and Technology Development Journal - Natural Sciences, 1(6), 58-67.

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